32BIOCHEMICAL, HPLC, LC-MS ANALYSIS AND BIOLOGICAL ACTIVITIES OF METHANOL EXTRACT OF ALSTONIA SCHOLARISThe methanol extract from the plant Alstonia scholaris (A.scholaris) was biochemically analyzed for different bioactive components.LC-MS analysis showed the presence of compounds like lupeol (16%) and epicatechin (2%). Different biochemical properties of this plant extract were evaluated. It showed antioxidant and anticancer effect. It showed significant antimicrobial effect also against tested microbes. Antimicrobial activity against gram positive organisms such as Staphylococcus aureus MTCC 87and gram negative organisms like Escherichia coli MTTCC 41, Proteus vulgaris 426 (ATCC 6380), Pseudomonas auriginosa 424(ATCC 25619) and Candida albicans MTTCC 183(ATCC 2091) and found to have significant inhibitory effect. It showed a minimum inhibitory concentration of 63µg/ml against Staphylococcus aureus. The minimum inhibitory concentration obtained against the resistant strain Methicillin resistant Staphylococcus aureus was 124µg/ml. The synergistic effect in combination of the extract with antibiotics also tested and proved to have synergistic effect reducing the MIC value of the drug to two-foldtara_menon2003@yahoo.co.inResearch322013Methanol extract,Alstonia scholaris,LC-MS,Lupeol,EpicatechinK.M.Thara,Fathimatheu ZuhraDepartment of Biotechnology, University of Calicut, Kerala, India,Professor, Department of Life Sciences, University of Calicut, Kerala, India